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Aous Khalefa brings over a decade of experience in analytical chemistry, with a strong specialization in GC×GC-TOFMS/FID and LC/MS-MS techniques. He holds a bachelor’s degree in Agricultural Engineering and a master’s degree in Agra Economy from Homs University. Currently, he works as an Applications Specialist for Separation Science at LECO Europe, where he supports the development and application of advanced chromatographic solutions. Throughout his career, he has contributed to numerous projects and collaborations, building expertise in areas such as MOSH/MOAH analysis, pesticide residues, environmental contaminants, mycotoxins (including aflatoxins), and molecular biology techniques. His work is dedicated to advancing chromatographic methods to solve complex challenges in food safety, environmental protection, and chemical analysis.
Ms. Visuvalingam has nearly 15 years’ experience with Time-of-Flight Mass Spectrometry (TOFMS). Having earned her Bachelor of Applied Science ( Hons.) in Analytical Chemistry, she is leading the Separation Science Sales Department at LECO Instruments Malaysia. Over the years, she has collaborated with many research organizations and universities, deepening her knowledge in various applications such as food safety, environmental, metabolomics and petrochemicals. Her focus revolves around aligning, implementing, and executing strategic initiatives in a timely manner to achieve the organization’s global objective.
Mineral Oil Hydrocarbons (MOH), comprising MOSH (saturated) and MOAH (aromatic) fractions, pose significant safety concerns in food and consumer products due to their potential toxicity and widespread occurrence. Accurate detection and quantification are critical for effective risk assessment and regulatory compliance. In this this presentation we will explore the advanced capabilities of LECO’s Two-Dimensional Gas Chromatography coupled with Time-of-Flight Mass Spectrometry and Flame Ionization Detection (GC×GC-TOFMS/FID) in MOSH/MOAH analysis. By combining enhanced chromatographic resolution and dual detection modes, GC×GC-TOFMS/FID enables precise separation, identification, and quantification of complex hydrocarbon mixtures across diverse matrices.

Food contamination with mineral oil hydrocarbons (MOH) has been a growing concern since 2012, when the European Food Safety Authority (EFSA) flagged them as a potential health concern .

The current analytical method for the quantitative determination of Mineral Oil Saturated Hydrocarbons (MOSH) and Mineral Oil Aromatic Hydrocarbons (MOAH) is LC-GC-FID, but the chromaticagraphic profile obtained is too often just a hump of unresolved substances.

LECO’s Pegasus BT 4D GCxGC-TOFMS system was used to review the complexity of contaminated food samples, providing superior chromatographic resolution and identification capabilities compared to the LC-GC-FID method.
September 10 – 11, 2025 | Royal Ambarrukmo Yogyakarta